The viral mmtv promoter directed expression of cre recombinase in the secretory epithelium of the mammary gland, the salivary gland, seminal vesicle, skin, erythrocytes, b and t cells. Mmtv was formerly known as bittner virus, and previously the milk factor, referring to the extrachromosomal vertical transmission of murine breast cancer by adoptive nursing, demonstrated in 1936, by john joseph bittner while working at the. The effect of mammary glandspecific expression of the polyomavirus middle t antigen was examined by establishing lines of transgenic mice that carry the middle t oncogene under the transcriptional control of the mouse mammary tumor virus promoterenhancer. The transformationspecific characteristics of spontaneous t cells leukemias of dba2 mice ml cells will be studied, and the possible leukemogenic activity of mmtv investigated. In 1997 we generated three distinct mouse lines carrying an identical mmtvcre transgene lines a, d and f.
Mmtvcre mice line d have been used in breeding, as this line has minimal effects on mammary gland development compared to other lines23. There is substantial evidence that a virus homologous to mouse mammary tumor virus mmtv may have a role in human breast cancer. Crelox basics generating knockout mice webinar 17mar2016. These mmtvcreline a mice express a transgene containing cre recombinase with widespread expression, including the female germline, under control the.
Preliminary experiments had demonstrated efficient deletion of the loxpflanked transcription stop site of the rosa26 allele and, thereby, expression of. The present study indicates that those who developed breast cancer associated with an mmtvlike virus had this virus in their noncancerous breast tissues years before the cancer developed. Rosa26 compound mice supplemental figure 4a, indicating that the mmtvcre line d transgene directs. If your answer is yes, please provide reference or contact details for approaching the owner. These mmtvcre line a mice express a transgene containing cre recombinase with widespread expression, including the female germline, under control the mmtv ltr promoter. Over the past decade several distinct transgenic mouse strains expressing cre recombinase in mammary epithelium have been established. A highly efficient ligandregulated cre recombinase mouse line shows that loxp recombination is position dependent. Mouse mammary tumor virus mmtv is a milktransmitted retrovirus like the htl viruses, hi viruses, and blv.
Time to hit the track for the annual american door slammer nationals this is the 5th event for the team at loose rocker promotions and is another event to give back to their supporters. Expression of the wapcre transgene in eight different mouse lines. The py230 cell line is an epitheliallike murine mammary luminal tumor cell line that has properties similar to those of normal mouse mammary stem cells. These mmtvcre line d mice express a transgene containing cre recombinase with widespread expression but not in oocytes under control the mmtv ltr promoter. Multiple genetic changes are associated with mammary. Mmtv mouse models and the diagnostic values of mmtvlike. High levels of recombination have been detected in the virgin and lactating mammary gland, salivary gland, seminal vesicle, skin, and various cells of the immune system. In addition to the three mmtv cre lines described, six other cre lines were used to disrupt or activate genes selectively in mammary epithelium. Mouse mammary tumorlike virus mmtv is present in human. Download table expression of the wapcre transgene in eight different mouse lines from publication.
Germline mutations in the tumor suppressor gene brca1 predispose women to breast cancer, however somatic mutations in the gene are rarely. These mmtvcreline d mice express a transgene containing cre recombinase with widespread expression but not in oocytes under control the mmtv ltr. View yiyu dong, phds profile on linkedin, the worlds largest professional community. The worlds most comprehensive professionally edited abbreviations and acronyms database all trademarksservice marks referenced on this site are properties of their respective owners.
See the complete profile on linkedin and discover yiyus. Mouse mammary tumor virus mmtvwas reported for the first time in 1933 by the jackson memorial laboratory1. It was initially identified by studying inbred mouse strains characterized by a high incidence of mammary tumor2. In contrast, recombination mediated by cre under control of the wap gene promoter was largely restricted to the mammary gland but occasionally observed in the brain. This transgene directs the expression of cre recombinase under the control of the mouse mammary tumor virus mmtv long terminal repeat. High levels of recombination in the virgin and lactating mammary gland, salivary. Cre required to maintain line for future generations genotype of whole mouse. N2 enforced expression of cmyc has been shown to serve as an apoptotic stimulus in cultured cells. The int1 gene was the first to be implicated in tumorigenesis on this basis, when it was discovered to be the target for mouse mammary tumor virus mmtv proviral insertions in the majority of virusinduced mammary carcinomas in c3h mice nusse and varmus, 1982. The following strains are currently being distributed from the nci mouse repository.
Mmtv is listed in the worlds largest and most authoritative dictionary database of abbreviations and acronyms the free dictionary. Hd streaming it works on your tv,pc,mac,ipad,iphone or android phone. Nov 01, 1997 in contrast, recombination mediated by cre under control of the wap gene promoter was largely restricted to the mammary gland but occasionally observed in the brain. Please see the executive director for more details. Visit the ordering information page for more information on the process of ordering mouse models from the nci mouse repository.
If you are using a phone for annnouncement submission, scroll to the bottom of the page. Lactation defect in a widely used mmtvcre transgenic line of mice. These mmtvcreline d mice express a transgene containing cre recombinase with widespread expression but not in oocytes under control the mmtv ltr promoter. Youll find tvmc to be the easiest out of the box kodibased solution available. The following mmtvcre transgenic lines were used for a comparison by histological examination and lactational performance. Join highvoltage professional speaker dave daley, the monster motivator as he teams up with local entrepreneurs, business managers, and individuals who are making big things happen. French name was registered on august 1, 20 to obtain a provincial charter to operate a television production company that will focus on representing the filipino canadian community in montreal and broadcast a show called mabuhay montreal tv. Analysis of transgenic mice carrying both the mmtvcre and the reporter transgenes revealed recombination in every tissue. Basic cre lox mechanism strain types and breeding schemes o tissuespecific knockouts.
Nowadays, it is the best studied oncogenic virus, causing 95% of mammary cancer in mice3. Click on the common strain name to view details on that strain. Uncoupling cancer mutations reveals critical timing of p53 loss in sarcomagenesis. Looking for online definition of mmtv or what mmtv stands for. Leder a, pattengale pk, kuo a, stewart ta, leder p.
Mmtv gene products and transformation albert einstein. Mmtvcre double mutant mouse breast cancer model was generated. Dues paying members of mmtv are allowed to sponsor programming produced from outside the city so it can air on the melrose network. The viral mmtv promoter directed expression of cre recombinase in the secretory epithelium of the mammary gland, the salivary gland and in the female germline. Widespread cre expression including the female germline. All the strains are currently on a mixed background and have not been backcrossed to an inbred strain. These results show that transgenic mice with wap cre but not mmtv cre can be used as a powerful tool to study gene function in development and tumorigenesis in the mammary gland.
Lactation defect in a widely used mmtvcre transgenic line. To develop an ipsc sox9 reporter line for monitoring differentiation into sox9 expressing cells such as chondrocytes, cranial neural crest and sertoli cells, we used gene editing to introduce sequences encoding the tdtomato fluorescent protein into the sox9 locus. It was derived from a mammary adenocarcinoma that spontaneously arose in a mmtvpymy mouse mammary tumor virus promoter driven polyoma middle tantigen transgenic c57bl6 female mouse. Normal mammary gland development after mmtvcre mediated.
Py230 atcc crl3279 mus musculus mammary gland adenocarci. T1 cell death in mmtv cmyc transgenic mouse mammary tumors may not be typical apoptosis. Consequences of widespread deregulation of the cmyc gene in transgenic mice. Watch live ghana tv, adom tv, tv3 ghana, joy news and many more with muti tv. Thank you to everyone who joined the dope holiday hullabaloo. Com xvideos wife tells cuck black cock is the best free. Jci ras and pi3kdependent breast tumorigenesis in mice. Efficient replication of mmtv occurs predominantly in the alveolar epithelial cells of the mammary gland. If you missed it, theres still time today to donate online at mmtv to the melrose emergency fund, and be entered in the dope holiday raffle. Initiation of metastatic breast carcinoma by targeting of the. Expression of the int1 gene in transgenic mice is associated.
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